05988933

j

05988933 Nekouzadeh, Ali Genin, Guy M. Quantification of fibre polymerization through Fourier space image analysis. Proc. R. Soc. Lond., Ser. A, Math. Phys. Eng. Sci. 467, No. 2132, 2310-2329 (2011). 2011 The Royal Society, London EN fibre length measurement cellular mechanics automated image processing

doi:10.1098/rspa.2010.0623

Summary: Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.