Summary: With rapid development of Internet, information and resource of Web academic database is great and explosive growth, so it is difficult to quickly and accurately obtain information which meets individual user’s needs. Web personalized services can effectively solve the problem of information overload problem and alleviate user’s cognitive burden. How to predict user interest is a key issue in Web personalized services. First, this paper proposes concepts of user knowledge unit and user knowledge flow that represents user short-term interest and long-term interest respectively. Second, existing methods have some defects which can’t be sensitive to perceive user interest change and accurately predict user real-time interest; we put forward Collaborative Time Weight (CTW) and Collaborative Relation Weight (CRW) to solve those problems. Meanwhile the prediction algorithm for user real-time interest is proposed based on collaborative filtering and interactive computing. Finally, experimental results demonstrate that our method can capture user real-time interests accurately and alleviate the user’s cognitive burden effectively.